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Patrick's father Cecil shows him the family "stache stash", which is where the Star family's ancestors stored their mustaches. Cecil offers Patrick to wear any of the mustaches, with the exception of one. After Cecil leaves the room, Patrick proceeds to try on various mustaches until he sees the mustache that Cecil didn't want him to wear. Patrick puts on the mustache and it causes him to become evil. He then kidnaps Squidina, Bunny, and GrandPat, and ties them to railroad tracks.
On The Patrick Show!, Patrick does a segment with Rube Goldfish where he asks him his thoughts on trains, and after Rube states that he lives trains, Patrick proceeds to have him tied up to the tracks, and then proceeds to tie up the rest of his audience to the tracks as well, with him planning on running over everyone with his train.
After Cecil gets on the train to save everyone, he finds Patrick and asks him to take off the mustache, only for Patrick's mustache to punch him instead. Patrick and Cecil's mustaches get into a fight, and Cecil's mustache eventually ties up Patrick, which allows Cecil to shave off Patrick's mustache.
Patrick finally returns to his normal self, but the train is still heading towards the tracks. Cecil eventually manages to stop the train in by using the force of his mustache to stop the train's momentum. Cecil gets his family ready for a photo after they are untied. Rube tries to take the picture, but said picture only gets the bottom half of the family because of Rube getting run over by a train.