Villains who appear in the Super Mario franchise, including the original Super Mario games, the Mario RPG games, and other Super Mario games, as well as cartoons, anime, manga, movies, and tabletop games based on the video games.

Villains from the Wario and Donkey Kong franchises who don't fight Mario such as the Shake King should not be in this category as this is only for characters who have officially fought Mario. Instead, they should be in their respective categories for their own series.