Mrs. Colambo, or just "the missus" as she's often called, is the wife of Colambo on Sesame Street. Like the spouse of Columbo, she is never seen on camera, but frequently mentioned.

According to Colambo in Episode 3468, she particularly loves being told what the sponsors of the day were. A pie personally baked by her has a plum stolen in "The Case of the Plum Plunder"; as punishment, Colambo tells perpetrator Little Jack Horner that he'll have to explain to her what happened to the pie. She also loves dogs ("The Case of the Missing Pig") and is always telling him, "Colambo, cooperate, work together toward a common goal, put your heads together" (Episode 3129). However, even Mrs. Colambo has no idea why her wooly husband is always leaving and coming back to ask another question (Episode 3100).