File:Computational-Analysis-of-the-Spatiotemporal-Coordination-of-Polarized-PI3K-and-Rac1-Activities-in-pone.0021293.s008.ogv
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Computational-Analysis-of-the-Spatiotemporal-Coordination-of-Polarized-PI3K-and-Rac1-Activities-in-pone.0021293.s008.ogv (Ogg Theora video file, length 7.8 s, 349 × 148 pixels, 264 kbps, file size: 252 KB)
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[edit]DescriptionComputational-Analysis-of-the-Spatiotemporal-Coordination-of-Polarized-PI3K-and-Rac1-Activities-in-pone.0021293.s008.ogv |
English: The distribution of PI3K activity in a representative MEF under PDGF stimulation. The fluorescent images representing the subcellular distribution of PI3K activity were overlaid on top of the DIC images of the MEF cells under PDGF stimulation. The MEFs were seeded on FN strips in the horizontal direction. The cell expressing PH-Akt-GFP was highlighted in pseudo-color among other cells without PH-Akt-GFP expression. PDGF stimulation induced a significant increase of free end dynamics accompanied by an increase of PH-Akt-GFP fluorescence (PI3K activity). |
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Source | Movie S1 from Lu S, Kim T, Chen C, Ouyang M, Seong J, Liao X, Wang Y (2011). "Computational Analysis of the Spatiotemporal Coordination of Polarized PI3K and Rac1 Activities in Micro-Patterned Live Cells". PLOS ONE. DOI:10.1371/journal.pone.0021293. PMID 21738630. PMC: 3124492. | ||
Author | Lu S, Kim T, Chen C, Ouyang M, Seong J, Liao X, Wang Y | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 22:12, 30 October 2012 | 7.8 s, 349 × 148 (252 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Lu S, Kim T, Chen C, Ouyang M, Seong J, Liao X, Wang Y |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | The distribution of PI3K activity in a representative MEF under PDGF stimulation. The fluorescent images representing the subcellular distribution of PI3K activity were overlaid on top of the DIC images of the MEF cells under PDGF stimulation. The MEFs were seeded on FN strips in the horizontal direction. The cell expressing PH-Akt-GFP was highlighted in pseudo-color among other cells without PH-Akt-GFP expression. PDGF stimulation induced a significant increase of free end dynamics accompanied by an increase of PH-Akt-GFP fluorescence (PI3K activity). |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2011 |