Line C is a line designation used in several public transport systems
- C (New York City Subway service), a subway route in New York City, United States
- C (Los Angeles Railway), former streetcar service In Los Angeles, US
- C (S-train), a commuter rail line in Copenhagen, Denmark
- C (AC Transit), a bus route in the San Francisco Bay
- C Line (Los Angeles Metro), a light rail line in Los Angeles County, California
- C Line (RTD), a light rail line in Denver, Colorado, United States
- Line C (Prague Metro), a metro line in Prague, Czech Republic
- Line C (Buenos Aires Underground), a metro line in Buenos Aires, Argentina
- Line C (Rome Metro), a metro line in Rome, Italy
- Avenue C Line (Manhattan), a bus route in New York City, United States
- Bordeaux Tramway line C, a tram route in Bordeaux, France
- Lyon Metro Line C, a metro line in Lyon, France
- Metro C Line (Minnesota), a rapid bus route in Minneapolis, United States
- RapidRide C Line, a bus route in Seattle, United States
- RER C, a commuter rail line in Paris, France