Castor and Pollux may refer to:
- Castor and Pollux, in Greek and Roman mythology, the twin sons of Lēda and Zeus/Tyndareus, who were transformed into the constellation Gemini
- Castor and Pollux, two stars in the constellation Gemini
- Castor and Pollux (elephants), two elephants kept at the zoo of the Jardin d'Acclimatation or Jardin des Plantes in Paris
- Castor and Pollux (Prado), an ancient Roman sculptural group
- Castor et Pollux, an opera by Jean-Philippe Rameau
- Castor and Pollux River, in Nunavut, Canada
- Castor and Pollux, a composition by Harry Partch
- Temple of Castor and Pollux, in the Roman Forum, Rome
- Castor (Zwillinge) and Pollux (Zwillinge), twin mountain peaks in the Pennine Alps on the Italian/Swiss border
- Castor and Pollux, two summits in Yellowstone National Park in Wyoming, USA
- Castor and Pollux (269 tons) was a British ship built at Teignmouth in 1790
- Castor and Pollux Troy, characters in the 1997 film Face/Off
- Castor & Pollux Natural Petworks, an American company founded in 1999 that manufactures organic and natural foods for cats and dogs