Agnus Dei generally refers to a liturgical prayer in honor of the Lamb of God, as used in Christian theology.
Agnus Dei may also refer to:
- Lamb of God, Agnus Dei in Latin, a term in Christian theology
- Agnus Dei, liturgical music which accompanies the prayer as part of a Mass setting
- Agnus Dei (Barber), a 1967 choral composition
- "Agnus Dei", a song from the Michael W. Smith album Go West Young Man
- Agnus Dei (film), a film by Anne Fontaine
- Agnus Dei (Zurbarán), a painting
- Agnus Dei Community, a religious community