ASRGL1
L-asparaginaza je enzim koji je kod ljudi kodiran genom ASRGL1.[5]
Aminokiselinska sekvenca
[uredi | uredi izvor]Dužina polipeptidnog lanca je 308 aminokiselina, a molekulska težina 32.055 Da.[6].
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MNPIVVVHGG | GAGPISKDRK | ERVHQGMVRA | ATVGYGILRE | GGSAVDAVEG | ||||
| AVVALEDDPE | FNAGCGSVLN | TNGEVEMDAS | IMDGKDLSAG | AVSAVQCIAN | ||||
| PIKLARLVME | KTPHCFLTDQ | GAAQFAAAMG | VPEIPGEKLV | TERNKKRLEK | ||||
| EKHEKGAQKT | DCQKNLGTVG | AVALDCKGNV | AYATSTGGIV | NKMVGRVGDS | ||||
| PCLGAGGYAD | NDIGAVSTTG | HGESILKVNL | ARLTLFHIEQ | GKTVEEAADL | ||||
| SLGYMKSRVK | GLGGLIVVSK | TGDWVAKWTS | TSMPWAAAKD | GKLHFGIDPD | ||||
| DTTITDLP |
Funkcija
[uredi | uredi izvor]Protein ASRGL1 sastoji se od 308 aminokiselina i aktivira se automatskim cijepanjem na aminokiselini 168, kako bi formirao alfa- i beta-lanac, koji se može dimerizirati u heterodimer.[7] Enzim ASRGL1 ima aktivnost i L-asparaginaze i beta-aspartil peptidaze i može biti uključen u proizvodnju L-aspartata, koji može djelovati kao ekscitacijski neurotransmiter u nekim regijama mozga.
Prema analizi profila i transkriptomike zasnovanoj na antitijelima, protein ASRGL1 prisutan je u svim analiziranim ljudskim tkivima, s najvećom ekspresijom u mozgu, u ženskim tkivima kao što su grlić materice i jajovod i muškom tkivu kao u sjemenicima.[8] Na osnovu konfokusne mikroskopije, ASRGL1 je uglavnom lokaliziran na mikrotubulama.[9]
Klinički značaj
[uredi | uredi izvor]ASRGL1 je visoko eksprimiran u normalnom endometriju i različito izražen u karcinomu endometrija. Gubitak ekspresije ASRGL1 nepovoljna je prognostička značajka za pacijente s karcinomom endometrija.[10][11]
Reference
[uredi | uredi izvor]- ^ a b c GRCh38: Ensembl release 89: ENSG00000162174 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000024654 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Entrez Gene: ASRGL1 asparaginase like 1".
- ^ "UniProt, Q7L266". Pristupljeno 16. 8. 2021.
- ^ Li, Wenzong; Cantor, Jason R.; Yogesha, S. D.; Yang, Shirley; Chantranupong, Lynne; Liu, June Qingxia; Agnello, Giulia; Georgiou, George; Stone, Everett M. (16. 11. 2012). "Uncoupling Intramolecular Processing and Substrate Hydrolysis in the N-Terminal Nucleophile Hydrolase hASRGL1 by Circular Permutation". ACS Chemical Biology. 7 (11): 1840–1847. doi:10.1021/cb300232n. ISSN 1554-8929. PMC 3514461. PMID 22891768.
- ^ "Tissue expression of ASRGL1 - Summary - The Human Protein Atlas". www.proteinatlas.org. Pristupljeno 3. 10. 2017.
- ^ "Cell atlas - ASRGL1 - The Human Protein Atlas". www.proteinatlas.org. Arhivirano s originala, 3. 1. 2019. Pristupljeno 3. 10. 2017.
- ^ "Expression of ASRGL1 in endometrial cancer - The Human Protein Atlas". www.proteinatlas.org. Pristupljeno 3. 10. 2017.
- ^ Edqvist, Per-Henrik D.; Huvila, Jutta; Forsström, Björn; Talve, Lauri; Carpén, Olli; Salvesen, Helga B.; Krakstad, Camilla; Grénman, Seija; Johannesson, Henrik (2015). "Loss of ASRGL1 expression is an independent biomarker for disease-specific survival in endometrioid endometrial carcinoma". Gynecologic Oncology. 137 (3): 529–537. doi:10.1016/j.ygyno.2015.03.055. PMID 25858696.
Dopunska literatura
[uredi | uredi izvor]- Kim JM, Lee KH, Jeon YJ, Oh JH, Jeong SY, Song IS, Kim JM, Lee DS, Kim NS (2006). "Identification of genes related to Parkinson's disease using expressed sequence tags". DNA Res. 13 (6): 275–86. doi:10.1093/dnares/dsl016. PMID 17213182.
- Kimura K, Wakamatsu A, Suzuki Y, Ota T, Nishikawa T, Yamashita R, Yamamoto J, Sekine M, Tsuritani K, Wakaguri H, Ishii S, Sugiyama T, Saito K, Isono Y, Irie R, Kushida N, Yoneyama T, Otsuka R, Kanda K, Yokoi T, Kondo H, Wagatsuma M, Murakawa K, Ishida S, Ishibashi T, Takahashi-Fujii A, Tanase T, Nagai K, Kikuchi H, Nakai K, Isogai T, Sugano S (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
- Suzuki Y, Yamashita R, Shirota M, Sakakibara Y, Chiba J, Mizushima-Sugano J, Nakai K, Sugano S (2004). "Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions". Genome Res. 14 (9): 1711–8. doi:10.1101/gr.2435604. PMC 515316. PMID 15342556.
- Bush LA, Herr JC, Wolkowicz M, Sherman NE, Shore A, Flickinger CJ (2002). "A novel asparaginase-like protein is a sperm autoantigen in rats". Mol. Reprod. Dev. 62 (2): 233–47. doi:10.1002/mrd.10092. PMID 11984834. S2CID 32206911.
- Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
- Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Vanjski linkovi
[uredi | uredi izvor]- ASRGL1 expression in the Human Protein Atlas
- Lokacija ljudskog genoma ASRGL1 i stranica sa detaljima o genu ASRGL1 u UCSC Genome Browseru.