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Comparative Study
. 1981 Jun;32(3):1249-53.
doi: 10.1128/iai.32.3.1249-1253.1981.

Interaction of Leishmania donovani promastigotes with human monocyte-derived macrophages: parasite entry, intracellular survival, and multiplication

R D Pearson, R Romito, P H Symes, J L Harcus
Comparative Study

Interaction of Leishmania donovani promastigotes with human monocyte-derived macrophages: parasite entry, intracellular survival, and multiplication

R D Pearson et al. Infect Immun. 1981 Jun.

Abstract

Leishmania donovani promastigotes were incubated with human monocyte-derived macrophages in vitro to assess the role of macrophages in the early stage of visceral leishmaniasis. Adherent mononuclear cells, obtained from nonimmune human donors, were cultivated on glass cover slips for 5 days and then incubated with axenically grown promastigotes in the presence of heat-inactivated autologous serum. Promastigotes attached to macrophages with either their flagellar or aflagellar ends, and macrophage pseudopodia formed around them. Intracellular parasites were identified within phagocytic vacuoles by electron microscopy, and the parasites assumed a form similar to that of amastigotes obtained from infected hamster spleens. Initially, 67 +/- 5% of the macrophages were infected with a mean of 4.2 +/- 0.7 parasites per infected cell. After 6 days of incubation, 79 +/- 7% of the macrophages were infected with 15.9 +/- 3.2 parasites per infected cell. The total number of parasites per monolayer increased from 4.8 +/- 0.8 x 10(5) to 1.8 +/- 0.4 x 10(6) (P less than 0.05). Dividing parasites were identified in macrophage vacuoles by electron microscopy. Human monocyte-derived macrophage vacuoles by electron microscopy. Human monocyte-derived macrophages can phagocytize promastigotes, allow the conversion of promastigotes to an amastigote-like state, and support intracellular multiplication.

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References

    1. Am J Trop Med Hyg. 1972 Nov;21(6):873-9 - PubMed
    1. J Parasitol. 1976 Jun;62(3):360-5 - PubMed
    1. Ann Trop Med Parasitol. 1977 Sep;71(3):295-312 - PubMed
    1. J Exp Med. 1978 Feb 1;147(2):515-30 - PubMed
    1. Experientia. 1978 May 15;34(5):598-9 - PubMed

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