In vitro activation of the contact (Hageman factor) system of plasma by heparin and chondroitin sulfate E
- PMID: 6202346
In vitro activation of the contact (Hageman factor) system of plasma by heparin and chondroitin sulfate E
Abstract
A large number of negatively charged macromolecules, including DNA, glycosaminoglycans, and proteoglycans, were tested as possible activators of the contact (Hageman factor) system in vitro. Activation was assessed by conversion of prekallikrein to kallikrein, as determined by amidolytic assay and by cleavage of 125I-Hageman factor into 52,000- and 28,000-dalton fragments. Of particular interest to these studies, heparin proteoglycan and glycosaminoglycan from rat peritoneal mast cells, and squid chondroitin sulfate E, which is representative of the glycosaminoglycan from cultured mouse bone marrow derived mast cells, induced the reciprocal activation between Hageman factor and prekallikrein. In addition, naturally occurring heparin glycosaminoglycans from pig mucosa, bovine lung, and rat mast cells also induced activation. In contrast, native connective tissue matrix glycosaminoglycans and proteoglycans from several sources were inactive, although when one such chondroitin sulfate was further sulfated in vitro, it gained activity. When the negative charge of the activating agents was blocked by the addition of hexadimethrine bromide, the cleavage of 125I-Hageman factor in the presence of prekallikrein was prevented. The active negatively charged macromolecules induced cleavage of 125I-high molecular weight kininogen in normal plasma but not in Hageman factor-deficient or prekallikrein-deficient plasmas. Reconstitution of prekallikrein-deficient plasma with purified prekallikrein restored the kininogen cleavage upon addition of the active proteoglycans. These results suggest that both heparin from connective tissue mast cells and highly sulfated chondroitin sulfate E from cultured mouse bone marrow derived mast cells (which are considered synonomous with mucosal mast cells) could activate the contact system of plasma subsequent to an activation secretion response.
Similar articles
-
Mast cell derived heparin activates the contact system: a link to kinin generation in allergic reactions.Clin Exp Allergy. 1997 Jun;27(6):653-63. Clin Exp Allergy. 1997. PMID: 9208186
-
A different cleavage site for high molecular weight kininogen in vivo following intravenous injection of dextran sulfate in the rabbit.Circ Res. 1986 Apr;58(4):595-604. doi: 10.1161/01.res.58.4.595. Circ Res. 1986. PMID: 2421941
-
The binding and cleavage characteristics of human Hageman factor during contact activation. A comparison of normal plasma with plasmas deficient in factor XI, prekallikrein, or high molecular weight kininogen.J Clin Invest. 1977 Jun;59(6):1167-75. doi: 10.1172/JCI108741. J Clin Invest. 1977. PMID: 864009 Free PMC article.
-
Molecular assembly in the contact phase of the Hageman factor system.Am J Med. 1979 Oct;67(4):657-64. doi: 10.1016/0002-9343(79)90253-5. Am J Med. 1979. PMID: 386794 Review.
-
Assembly of the human plasma kinin-forming cascade along the surface of vascular endothelial cells.Int Arch Allergy Immunol. 1995 May-Jun;107(1-3):93-4. doi: 10.1159/000236941. Int Arch Allergy Immunol. 1995. PMID: 7613224 Review.
Cited by
-
Mast cell degranulation and bradykinin-induced angioedema - searching for the missing link.Front Immunol. 2024 May 15;15:1399459. doi: 10.3389/fimmu.2024.1399459. eCollection 2024. Front Immunol. 2024. PMID: 38812508 Free PMC article. Review.
-
The bradykinin-forming cascade in anaphylaxis and ACE-inhibitor induced angioedema/airway obstruction.Front Allergy. 2024 Jan 25;5:1302605. doi: 10.3389/falgy.2024.1302605. eCollection 2024. Front Allergy. 2024. PMID: 38332896 Free PMC article. Review.
-
Hemodialysis-Related Complement and Contact Pathway Activation and Cardiovascular Risk: A Narrative Review.Kidney Med. 2021 Jun 9;3(4):607-618. doi: 10.1016/j.xkme.2021.04.006. eCollection 2021 Jul-Aug. Kidney Med. 2021. PMID: 34401728 Free PMC article. Review.
-
Defective NET clearance contributes to sustained FXII activation in COVID-19-associated pulmonary thrombo-inflammation.EBioMedicine. 2021 May;67:103382. doi: 10.1016/j.ebiom.2021.103382. Epub 2021 May 14. EBioMedicine. 2021. PMID: 34000623 Free PMC article.
-
The Mast Cell, Contact, and Coagulation System Connection in Anaphylaxis.Front Immunol. 2017 Jul 26;8:846. doi: 10.3389/fimmu.2017.00846. eCollection 2017. Front Immunol. 2017. PMID: 28798744 Free PMC article. Review.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical