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. 2023 Oct;52(5):290-293.
doi: 10.1111/jmp.12674. Epub 2023 Sep 1.

Characterizing the phenotypic and genetic changes of pre-epidemic HIV-2 group F virus following serial passage in humanized mice

James Z Curlin  1   2 , Kimberly Schmitt  1 , Leila Remling-Mulder  1 , Corina Valencia Tibbitts  3 , Shelby O'Connor  3 , Preston Marx  4   5 , Ramesh Akkina  1
Affiliations

Characterizing the phenotypic and genetic changes of pre-epidemic HIV-2 group F virus following serial passage in humanized mice

James Z Curlin et al. J Med Primatol. 2023 Oct.

Abstract

HIV-2 Group F virus with an origin in NHPs was isolated from only two individuals. Two serial passages in hu-mice showed increased viral loads, CD4+ T cell decline and nonsynonymous genetic changes showing its capacity for further evolution, and spread in the human.

Keywords: HIV-2 group F; hu-HSC mice and HIV evolution; humanized mice; serial passaging; viral evolution.

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Figures

Figure 1.
Figure 1.. Infection with HIV-2 Group F results in persistent viremia and CD4+ T cell decline in hu-mice by passage 2.
Plasma viral loads (A.) and CD4+ T cell levels (B.) are presented as the mean ± SD. Statistically significant differences were seen between the first and second passage plasma viral loads (****p < 0.0001) and the CD4+ T cell decline between uninfected and infected passage 2 (**p < 0.005).
Figure 2.
Figure 2.. Nonsynonymous SNPs with ≥50% average endpoint frequency following two serial passages of HIV-2 Group F in hu-mice.
Variant frequency is indicated by the red scale and required ≥100 read depth of coverage.
See this image and copyright information in PMC

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