Enhancement of Breast Cancer Cell Aggressiveness by lncRNA H19 and its Mir-675 Derivative: Insight into Shared and Different Actions
- PMID: 32610610
- PMCID: PMC7407157
- DOI: 10.3390/cancers12071730
Enhancement of Breast Cancer Cell Aggressiveness by lncRNA H19 and its Mir-675 Derivative: Insight into Shared and Different Actions
Abstract
Breast cancer is a major public health problem and the leading world cause of women death by cancer. Both the recurrence and mortality of breast cancer are mainly caused by the formation of metastasis. The long non-coding RNA H19, the precursor of miR-675, is involved in breast cancer development. The aim of this work was to determine the implication but, also, the relative contribution of H19 and miR-675 to the enhancement of breast cancer metastatic potential. We showed that both H19 and miR-675 increase the invasive capacities of breast cancer cells in xenografted transgenic zebrafish models. In vitro, H19 and miR-675 enhance the cell migration and invasion, as well as colony formation. H19 seems to induce the epithelial-to-mesenchymal transition (EMT), with a decreased expression of epithelial markers and an increased expression of mesenchymal markers. Interestingly, miR-675 simultaneously increases the expression of both epithelial and mesenchymal markers, suggesting the induction of a hybrid phenotype or mesenchymal-to-epithelial transition (MET). Finally, we demonstrated for the first time that miR-675, like its precursor H19, increases the stemness properties of breast cancer cells. Altogether, our data suggest that H19 and miR-675 could enhance the aggressiveness of breast cancer cells through both common and different mechanisms.
Keywords: H19 gene; LncRNA; breast cancer; cancer stem cell; miR-675; tumoral progression.
Conflict of interest statement
The authors declare no conflicts of interest.
Figures










Similar articles
-
Long noncoding RNA H19 acts as a miR-340-3p sponge to promote epithelial-mesenchymal transition by regulating YWHAZ expression in paclitaxel-resistant breast cancer cells.Environ Toxicol. 2020 Sep;35(9):1015-1028. doi: 10.1002/tox.22938. Epub 2020 May 18. Environ Toxicol. 2020. PMID: 32420678
-
Oncofetal H19 RNA promotes tumor metastasis.Biochim Biophys Acta. 2014 Jul;1843(7):1414-26. doi: 10.1016/j.bbamcr.2014.03.023. Epub 2014 Apr 2. Biochim Biophys Acta. 2014. PMID: 24703882
-
The Effect of LncRNA H19/miR-194-5p Axis on the Epithelial-Mesenchymal Transition of Colorectal Adenocarcinoma.Cell Physiol Biochem. 2018;50(1):196-213. doi: 10.1159/000493968. Epub 2018 Oct 2. Cell Physiol Biochem. 2018. PMID: 30278464
-
The role of long non-coding RNA H19 in breast cancer.Oncol Lett. 2020 Jan;19(1):7-16. doi: 10.3892/ol.2019.11093. Epub 2019 Nov 14. Oncol Lett. 2020. PMID: 31897110 Free PMC article. Review.
-
Regulation of Human Breast Cancer by the Long Non-Coding RNA H19.Int J Mol Sci. 2017 Nov 3;18(11):2319. doi: 10.3390/ijms18112319. Int J Mol Sci. 2017. PMID: 29099749 Free PMC article. Review.
Cited by
-
The Use of Zebrafish Xenotransplant Assays to Analyze the Role of lncRNAs in Breast Cancer.Front Oncol. 2021 May 27;11:687594. doi: 10.3389/fonc.2021.687594. eCollection 2021. Front Oncol. 2021. PMID: 34123857 Free PMC article. Review.
-
Targeting epigenetic alterations in cancer stem cells.Front Mol Med. 2022 Sep 20;2:1011882. doi: 10.3389/fmmed.2022.1011882. eCollection 2022. Front Mol Med. 2022. PMID: 39086963 Free PMC article. Review.
-
One Host-Multiple Applications: Zebrafish (Danio rerio) as Promising Model for Studying Human Cancers and Pathogenic Diseases.Int J Mol Sci. 2022 Sep 6;23(18):10255. doi: 10.3390/ijms231810255. Int J Mol Sci. 2022. PMID: 36142160 Free PMC article. Review.
-
Splice and Dice: Intronic microRNAs, Splicing and Cancer.Biomedicines. 2021 Sep 19;9(9):1268. doi: 10.3390/biomedicines9091268. Biomedicines. 2021. PMID: 34572454 Free PMC article. Review.
-
Ginsenoside Rd inhibits migration and invasion of tongue cancer cells through H19/miR-675-5p/CDH1 axis.J Appl Oral Sci. 2022 Sep 2;30:e20220144. doi: 10.1590/1678-7757-2022-0144. eCollection 2022. J Appl Oral Sci. 2022. PMID: 36074434 Free PMC article.
References
-
- Adriaenssens E., Dumont L., Lottin S., Bolle D., Leprêtre A., Delobelle A., Bouali F., Dugimont T., Coll J., Curgy J.J. H19 overexpression in breast adenocarcinoma stromal cells is associated with tumor values and steroid receptor status but independent of p53 and Ki-67 expression. Am. J. Pathol. 1998;153:1597–1607. doi: 10.1016/S0002-9440(10)65748-3. - DOI - PMC - PubMed
Grants and funding
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous