AP2 clathrin adaptor complex, but not AP1, controls the access of the major histocompatibility complex (MHC) class II to endosomes
- PMID: 15749704
- DOI: 10.1074/jbc.M501357200
AP2 clathrin adaptor complex, but not AP1, controls the access of the major histocompatibility complex (MHC) class II to endosomes
Abstract
Newly synthesized MHC II alpha- and beta-chains associated with the invariant chain chaperone (Ii) enter the endocytic pathway for Ii degradation and loading with peptides before transport to the cell surface. It is unclear how alphabetaIi complexes are sorted from the Golgi apparatus and directed to endosomes. However, indirect evidence tends to support direct transport involving the AP1 clathrin adaptor complex. Surprisingly, we show here that knocking down the production of AP1 by RNA interference did not affect the trafficking of alphabetaIi complexes. In contrast, AP2 depletion led to a large increase in surface levels of alphabetaIi complexes, inhibited their rapid internalization, and strongly delayed the appearance of mature MHC II in intracellular compartments. Thus, in the cell systems studied here, rapid internalization of alphabetaIi complexes via an AP2-dependent pathway represents a key step for MHC II delivery to endosomes and lysosomes.
Similar articles
-
Early endosomes are required for major histocompatiblity complex class II transport to peptide-loading compartments.Mol Biol Cell. 1999 Sep;10(9):2891-904. doi: 10.1091/mbc.10.9.2891. Mol Biol Cell. 1999. PMID: 10473634 Free PMC article.
-
Involvement of clathrin and AP-2 in the trafficking of MHC class II molecules to antigen-processing compartments.Proc Natl Acad Sci U S A. 2005 May 31;102(22):7910-5. doi: 10.1073/pnas.0502206102. Epub 2005 May 23. Proc Natl Acad Sci U S A. 2005. PMID: 15911768 Free PMC article.
-
Transient surface delivery of invariant chain-MHC II complexes via endosomes: a quantitative study.Traffic. 2002 Feb;3(2):133-46. doi: 10.1034/j.1600-0854.2002.030206.x. Traffic. 2002. PMID: 11929603
-
Conformational regulation of AP1 and AP2 clathrin adaptor complexes.Traffic. 2019 Oct;20(10):741-751. doi: 10.1111/tra.12677. Epub 2019 Aug 6. Traffic. 2019. PMID: 31313456 Free PMC article. Review.
-
Trafficking of MHC class II molecules in the late secretory pathway.Curr Opin Immunol. 2002 Feb;14(1):30-5. doi: 10.1016/s0952-7915(01)00295-3. Curr Opin Immunol. 2002. PMID: 11790530 Review.
Cited by
-
The ins and outs of MHC class II-mediated antigen processing and presentation.Nat Rev Immunol. 2015 Apr;15(4):203-16. doi: 10.1038/nri3818. Epub 2015 Feb 27. Nat Rev Immunol. 2015. PMID: 25720354 Free PMC article. Review.
-
Boosting immune response with the invariant chain segments via association with non-peptide binding region of major histocompatibility complex class II molecules.BMC Immunol. 2012 Sep 27;13:55. doi: 10.1186/1471-2172-13-55. BMC Immunol. 2012. PMID: 23016601 Free PMC article.
-
Major histocompatibility complex class II molecules promote human immunodeficiency virus type 1 assembly and budding to late endosomal/multivesicular body compartments.J Virol. 2006 Oct;80(19):9789-97. doi: 10.1128/JVI.01055-06. J Virol. 2006. PMID: 16973583 Free PMC article.
-
A specialized tyrosine-based endocytosis signal in MR1 controls antigen presentation to MAIT cells.J Cell Biol. 2022 Dec 5;221(12):e202110125. doi: 10.1083/jcb.202110125. Epub 2022 Sep 21. J Cell Biol. 2022. PMID: 36129434 Free PMC article.
-
AP-1 and ARF1 control endosomal dynamics at sites of FcR mediated phagocytosis.Mol Biol Cell. 2007 Dec;18(12):4921-31. doi: 10.1091/mbc.e07-04-0392. Epub 2007 Oct 3. Mol Biol Cell. 2007. PMID: 17914058 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
