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. 2005 Jan 14;120(1):15-20.
doi: 10.1016/j.cell.2004.12.035.

Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets

Benjamin P Lewis, Christopher B Burge, David P Bartel
Free article

Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets

Benjamin P Lewis et al. Cell. .
Free article

Abstract

We predict regulatory targets of vertebrate microRNAs (miRNAs) by identifying mRNAs with conserved complementarity to the seed (nucleotides 2-7) of the miRNA. An overrepresentation of conserved adenosines flanking the seed complementary sites in mRNAs indicates that primary sequence determinants can supplement base pairing to specify miRNA target recognition. In a four-genome analysis of 3' UTRs, approximately 13,000 regulatory relationships were detected above the estimate of false-positive predictions, thereby implicating as miRNA targets more than 5300 human genes, which represented 30% of our gene set. Targeting was also detected in open reading frames. In sum, well over one third of human genes appear to be conserved miRNA targets.

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