A genome annotation-driven approach to cloning the human ORFeome
- PMID: 15461802
- PMCID: PMC545604
- DOI: 10.1186/gb-2004-5-10-r84
A genome annotation-driven approach to cloning the human ORFeome
Abstract
We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones representing 70% of genes on human chromosome 22, whereas searching available cDNA clone collections found at best 48% from a single collection and 60% for all collections combined.
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