A genome annotation-driven approach to cloning the human ORFeome
- PMID: 15461802
- PMCID: PMC545604
- DOI: 10.1186/gb-2004-5-10-r84
A genome annotation-driven approach to cloning the human ORFeome
Abstract
We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones representing 70% of genes on human chromosome 22, whereas searching available cDNA clone collections found at best 48% from a single collection and 60% for all collections combined.
Figures



Similar articles
-
The full-ORF clone resource of the German cDNA Consortium.BMC Genomics. 2007 Oct 31;8:399. doi: 10.1186/1471-2164-8-399. BMC Genomics. 2007. PMID: 17974005 Free PMC article.
-
C. elegans ORFeome version 3.1: increasing the coverage of ORFeome resources with improved gene predictions.Genome Res. 2004 Oct;14(10B):2064-9. doi: 10.1101/gr.2496804. Genome Res. 2004. PMID: 15489327 Free PMC article.
-
Systematic recovery and analysis of full-ORF human cDNA clones.Genome Res. 2004 Oct;14(10B):2083-92. doi: 10.1101/gr.2473704. Genome Res. 2004. PMID: 15489330 Free PMC article.
-
From genome to proteome: developing expression clone resources for the human genome.Hum Mol Genet. 2006 Apr 15;15 Spec No 1:R31-43. doi: 10.1093/hmg/ddl048. Hum Mol Genet. 2006. PMID: 16651367 Review.
-
ORFeome cloning and systems biology: standardized mass production of the parts from the parts-list.Genome Res. 2004 Oct;14(10B):2001-9. doi: 10.1101/gr.2769804. Genome Res. 2004. PMID: 15489318 Review.
Cited by
-
A biomedically enriched collection of 7000 human ORF clones.PLoS One. 2008 Jan 30;3(1):e1528. doi: 10.1371/journal.pone.0001528. PLoS One. 2008. PMID: 18231609 Free PMC article.
-
Identification and characterization of a Golgi retention signal in feline coronavirus accessory protein 7b.J Gen Virol. 2017 Aug;98(8):2017-2029. doi: 10.1099/jgv.0.000879. Epub 2017 Jul 31. J Gen Virol. 2017. PMID: 28758629 Free PMC article.
-
Prohibitin: A Novel Molecular Player in KDEL Receptor Signalling.Biomed Res Int. 2015;2015:319454. doi: 10.1155/2015/319454. Epub 2015 May 10. Biomed Res Int. 2015. PMID: 26064897 Free PMC article.
-
Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression.BMC Biotechnol. 2004 Dec 14;4:32. doi: 10.1186/1472-6750-4-32. BMC Biotechnol. 2004. PMID: 15598350 Free PMC article.
-
p38 Molecular Targeting for Next-Generation Multiple Myeloma Therapy.Cancers (Basel). 2024 Jan 6;16(2):256. doi: 10.3390/cancers16020256. Cancers (Basel). 2024. PMID: 38254747 Free PMC article. Review.
References
-
- Reboul J, Vaglio P, Rual JF, Lamesch P, Martinez M, Armstrong CM, Li S, Jacotot L, Bertin N, Janky R, et al. C. elegans ORFeome version 1.1: experimental verification of the genome annotation and resource for proteome-scale protein expression. Nat Genet. 2003;34:35–41. doi: 10.1038/ng1140. - DOI - PubMed
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources