doi: 10.1016/0006-291x(92)90847-e.
Conjugal transfer of plasmid and transposon DNA from Escherichia coli into Porphyromonas gingivalis
Affiliations
- PMID: 1323280
- DOI: 10.1016/0006-291x(92)90847-e
Item in Clipboard
Conjugal transfer of plasmid and transposon DNA from Escherichia coli into Porphyromonas gingivalis
Biochem Biophys Res Commun.
.
Abstract
Using the broad host-range vector R751 to provide transfer functions, plasmid pVAL-1 and transposon Tn4351 were conjugally mobilized from Escherichia coli into Porphyromonas gingivalis. Transfer frequencies for both elements varied between 10(-6) and 10(-11), depending upon the recipient. The behavior of pVAL-1 and Tn4351 in P. gingivalis was essentially as described previously in Bacteroides spp. These data indicate that plasmid and transposon DNA can be conjugally transferred into P. gingivalis and that these elements can be used to genetically manipulate the organism in examining putative virulence determinants that may participate in the induction or exacerbation of periodontal disease.
Similar articles
-
Transposition of Tn4351 in Porphyromonas gingivalis.Plasmid. 1992 May;27(3):246-50. doi: 10.1016/0147-619x(92)90028-9. Plasmid. 1992. PMID: 1325062
-
The introduction of colonic-Bacteroides shuttle plasmids into Porphyromonas gingivalis: identification of a putative P. gingivalis insertion-sequence element.FEMS Microbiol Lett. 1992 May 15;72(1):75-81. doi: 10.1016/0378-1097(92)90492-7. FEMS Microbiol Lett. 1992. PMID: 1319376
-
Tn4351-generated non-haemolytic and/or non-pigmented mutants of Porphyromonas gingivalis.Microbios. 1998;95(380):35-44. Microbios. 1998. PMID: 9881462
-
Resistance factors in anaerobic bacteria.Scand J Infect Dis Suppl. 1986;49:56-63. Scand J Infect Dis Suppl. 1986. PMID: 3029859 Review.
-
[The application of a transponson, Tn4351, to an oral anaerobe, Porphyromonas gingivalis].Nihon Saikingaku Zasshi. 1998 Aug;53(3):509-18. doi: 10.3412/jsb.53.509. Nihon Saikingaku Zasshi. 1998. PMID: 9780735 Review. Japanese. No abstract available.
Cited by
-
Resistance of a Tn4351-generated polysaccharide mutant of Porphyromonas gingivalis to polymorphonuclear leukocyte killing.Infect Immun. 1995 Feb;63(2):393-401. doi: 10.1128/iai.63.2.393-401.1995. Infect Immun. 1995. PMID: 7822002 Free PMC article.
-
Development of techniques for the genetic manipulation of the gliding bacterium Cytophaga johnsonae.J Bacteriol. 1996 Feb;178(3):583-90. doi: 10.1128/jb.178.3.583-590.1996. J Bacteriol. 1996. PMID: 8550486 Free PMC article.
-
Illuminating the oral microbiome and its host interactions: tools and approaches for molecular microbiology studies.FEMS Microbiol Rev. 2023 Nov 1;47(6):fuac050. doi: 10.1093/femsre/fuac050. FEMS Microbiol Rev. 2023. PMID: 36549660 Free PMC article. Review.
-
Development of techniques to genetically manipulate members of the genera Cytophaga, Flavobacterium, Flexibacter, and Sporocytophaga.Appl Environ Microbiol. 1996 Aug;62(8):3017-22. doi: 10.1128/aem.62.8.3017-3022.1996. Appl Environ Microbiol. 1996. PMID: 8702294 Free PMC article.
-
Genetic tools for studying Capnocytophaga canimorsus.Appl Environ Microbiol. 2008 Oct;74(20):6369-77. doi: 10.1128/AEM.01218-08. Epub 2008 Aug 22. Appl Environ Microbiol. 2008. PMID: 18723653 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Miscellaneous
