C/EBP gamma has a stimulatory role on the IL-6 and IL-8 promoters
- PMID: 12177065
- DOI: 10.1074/jbc.M206224200
C/EBP gamma has a stimulatory role on the IL-6 and IL-8 promoters
Abstract
CCAAT/enhancer-binding protein gamma (C/EBP gamma) is an ubiquitously expressed member of the C/EBP family of transcription factors that has been shown to be an inhibitor of C/EBP transcriptional activators and has been proposed to act as a buffer against C/EBP-mediated activation. We have now unexpectedly found that C/EBP gamma dramatically augments the activity of C/EBP beta in lipopolysaccharide induction of the interleukin-6 and interleukin-8 promoters in a B lymphoblast cell line. This activating role for C/EBP gamma is promoter-specific, neither being observed in the regulation of a simple C/EBP-dependent promoter nor the TNF alpha promoter. C/EBP gamma activity also shows cell-type specificity with no activity observed in a macrophage cell line. Studies with chimeric C/EBP proteins implicate the formation of a heterodimeric leucine zipper between C/EBP beta and C/EBP gamma as the critical structural feature required for C/EBP gamma stimulatory activity. These findings suggest a unique role for C/EBP gamma in B cell gene regulation and, along with our previous observation of the ability of C/EBP basic region-leucine zipper domains to confer lipopolysaccharide inducibility of interleukin-6, suggest that the C/EBP leucine zipper domain has a role in C/EBP function beyond allowing dimerization between C/EBP family members.
Similar articles
-
The C/EBP bZIP domain can mediate lipopolysaccharide induction of the proinflammatory cytokines interleukin-6 and monocyte chemoattractant protein-1.J Biol Chem. 2000 May 26;275(21):16373-81. doi: 10.1074/jbc.M910269199. J Biol Chem. 2000. PMID: 10748205
-
The kappa B enhancer of the human interleukin-6 promoter is necessary and sufficient to confer an IL-1 beta and TNF-alpha response in transfected human cell lines: requirement for members of the C/EBP family for activity.J Interferon Cytokine Res. 1996 Oct;16(10):783-98. doi: 10.1089/jir.1996.16.783. J Interferon Cytokine Res. 1996. PMID: 8910763
-
Functional and physical associations between NF-kappa B and C/EBP family members: a Rel domain-bZIP interaction.Mol Cell Biol. 1993 Jul;13(7):3964-74. doi: 10.1128/mcb.13.7.3964-3974.1993. Mol Cell Biol. 1993. PMID: 8321203 Free PMC article.
-
CCAAT enhancer binding protein gamma (C/EBP-γ): An understudied transcription factor.Adv Biol Regul. 2022 May;84:100861. doi: 10.1016/j.jbior.2022.100861. Epub 2022 Jan 26. Adv Biol Regul. 2022. PMID: 35121409 Free PMC article. Review.
-
The "Janus" Role of C/EBPs Family Members in Cancer Progression.Int J Mol Sci. 2020 Jun 17;21(12):4308. doi: 10.3390/ijms21124308. Int J Mol Sci. 2020. PMID: 32560326 Free PMC article. Review.
Cited by
-
CCAAT/Enhancer-Binding Proteins in Fibrosis: Complex Roles Beyond Conventional Understanding.Research (Wash D C). 2022 Oct 3;2022:9891689. doi: 10.34133/2022/9891689. eCollection 2022. Research (Wash D C). 2022. PMID: 36299447 Free PMC article. Review.
-
C/EBPγ suppresses senescence and inflammatory gene expression by heterodimerizing with C/EBPβ.Mol Cell Biol. 2013 Aug;33(16):3242-58. doi: 10.1128/MCB.01674-12. Epub 2013 Jun 17. Mol Cell Biol. 2013. PMID: 23775115 Free PMC article.
-
CEBPG transcription factor correlates with antioxidant and DNA repair genes in normal bronchial epithelial cells but not in individuals with bronchogenic carcinoma.BMC Cancer. 2005 Oct 29;5:141. doi: 10.1186/1471-2407-5-141. BMC Cancer. 2005. PMID: 16255782 Free PMC article.
-
Hyperforin, the active component of St. John's wort, induces IL-8 expression in human intestinal epithelial cells via a MAPK-dependent, NF-kappaB-independent pathway.J Clin Immunol. 2004 Nov;24(6):623-36. doi: 10.1007/s10875-004-6248-z. J Clin Immunol. 2004. PMID: 15622447
-
Critical role for CCAAT/enhancer-binding protein β in immune complex-induced acute lung injury.J Immunol. 2012 Aug 1;189(3):1480-90. doi: 10.4049/jimmunol.1200877. Epub 2012 Jun 25. J Immunol. 2012. PMID: 22732594 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
