Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2002 Feb;40(2):325-9.
doi: 10.1128/JCM.40.02.325-329.2002.

Highly sensitive assay for human herpesvirus 8 antibodies that uses a multiple antigenic peptide derived from open reading frame K8.1

Lee L Lam  1 , Chou-Pong Pau, Sheila C Dollard, Philip E Pellett, Thomas J Spira
Affiliations

Highly sensitive assay for human herpesvirus 8 antibodies that uses a multiple antigenic peptide derived from open reading frame K8.1

Lee L Lam et al. J Clin Microbiol. 2002 Feb.

Abstract

The immunodominant region of the human herpesvirus 8 (HHV-8), the antibody-binding site of glycoprotein K8.1A, was mapped to the N-terminal region by using overlapping peptides and a residue replacement method. The main epitope was located within residues 44 to 56 (GQVYQDWL----C). Based on this information, we developed an enzyme immunoassay to detect HHV-8 antibodies in human sera using a four-branch multiple antigenic peptide as the antigen. The sensitivity and specificity of the assay were 96 and 99.4%, respectively. This assay should be useful for population-based, epidemiological studies of HHV-8 infection.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Serum reactivities of 12 overlapping peptides (Table 1) derived from HHV-8 gpK8.1A. Pools A, B, and C are serum pools containing four different randomly chosen specimens from 81 KS patients. NC is a normal control serum from a healthy blood donor. O.D., optical density.
FIG. 2.
FIG. 2.
Results of fine epitope mapping by the amino acid replacement method. Peptide sequences are listed in Table 2. Amino acid substitutions are indicated on the x axis. The serum reactivity of each peptide is compared to that of the wild-type (WT) peptide (P2). Black and white bars represent two different KS-positive specimens tested in this assay.
FIG. 3.
FIG. 3.
Extending the sequence of P2 toward the N terminus increases the analytical sensitivity with some sera. Serum A is a highly reactive specimen, while serum B is a weakly reactive specimen. O.D., optical density.
FIG. 4.
FIG. 4.
Typical serum reactivities of peptides PK8.1-MAP (▵), PK8.1 (•), and P2 (⧫) with two (a and b) KS-positive specimens. O.D., optical density.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Ablashi, D., L. Chatlynne, H. Cooper, D. Thomas, M. Yadav, A. W. Norhanom, A. K. Chandana, V. Churdboonchart, S. A. Kulpradist, M. Patnaik, K. Liegmann, R. Masood, M. Reitz, F. Cleghorn, A. Manns, P. H. Levine, C. Rabkin, R. Biggar, F. Jensen, P. Gill, N. Jack, J. Edwards, J. Whitman, and C. Boshoff. 1999. Seroprevalence of human herpesvirus-8 (HHV-8) in countries of Southeast Asia compared to the USA, the Caribbean and Africa. Br. J. Cancer 81:893-897. - PMC - PubMed
    1. Cesarman, E., Y. Chang, P. S. Moore, J. W. Said, and D. M. Knowles. 1995. Kaposi's sarcoma-associated herpesvirus-like DNA sequences in AIDS-related body-cavity-based lymphomas. N. Engl. J. Med. 332:1186-1191. - PubMed
    1. Chandran, B., C. Bloomer, S. R. Chan, L. Zhu, E. Goldstein, and R. Horvat. 1998. Human herpesvirus-8 ORF K8.1 gene encodes immunogenic glycoproteins generated by spliced transcripts. Virology 249:140-149. - PubMed
    1. Chandran, B., M. S. Smith, D. M. Koelle, L. Corey, R. Horvat, and E. Goldstein. 1998. Reactivities of human sera with human herpesvirus-8-infected BCBL-1 cells and identification of HHV-8-specific proteins and glycoproteins and the encoding cDNAs. Virology 243:208-217. - PubMed
    1. Chang, Y., E. Cesarman, M. S. Pessin, F. Lee, J. Culpepper, D. M. Knowles, and P. S. Moore. 1994. Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi's sarcoma. Science 266:1865-1869. - PubMed

Publication types