Detection of isolated tumor cells in bone marrow in early-stage breast carcinoma patients: comparison with preoperative clinical parameters and primary tumor characteristics
- PMID: 11751511
Detection of isolated tumor cells in bone marrow in early-stage breast carcinoma patients: comparison with preoperative clinical parameters and primary tumor characteristics
Abstract
PURPOSE/EXPERIMENTAL DESIGN: The importance of detection of disseminated isolated tumor cells (ITCs) in bone marrow (BM) is still not settled. BM aspirates from 920 patients with primary breast cancer were analyzed for tumor cells by standardized direct immunocytochemical analysis (ICC) of 2 x 10(6) mononuclear cells (MNCs) using anticytokeratin monoclonal antibody (AE1/AE3). Samples (637) were analyzed by negative immunomagnetic enrichment (IMS) followed by ICC (10 x 10(6) MNCs). Analyses of the primary tumor specimens have been performed, including histomorphology, tumor-node-metastasis (TNM) staging, grading, and immunohistochemical analyses.
Results: Of the patients with infiltrating carcinoma, 63% were node negative (N0) and 33%, node positive (N+). The results show the presence of tumor cells in 13.4% of the evaluable patients after direct ICC analysis. The presence of tumor cells correlated to the nodal- and tumor stage, showing BM positivity in 9.9% of the N0 cases and 20.6% in the N+ group (P < 0.0005), 11.2% of the stage T(1) were positive, and 15.0% and 22.6% were positive in the T(2) and T(3/4) groups, respectively (P = 0.013). No correlation between detection of ITC and detection of p53 and cathepsin D expression was found. Vascular invasion and c-erbB2 expression were associated with ITCs in BM (P = 0.045 and P = 0.024, respectively). Node-negative patients with estrogen receptor (ER)+ and/or progesterone receptor (PgR)+ tumors had lower frequency of ITCs than ER-/PgR- (P = 0.004). The use of negative IMS increased the frequency of positive BM by 63% (P < 0.0005).
Conclusions: The direct ICC detection of ITCs in BM correlated with primary tumor stage, nodal stage, vascular invasion, c-erbB2 expression, and ER/PgR status. Analysis of larger BM samples by negative IMS resulted in increased number of ITC-positive patients.
Similar articles
-
Detection of isolated tumor cells in bone marrow is an independent prognostic factor in breast cancer.J Clin Oncol. 2003 Sep 15;21(18):3469-78. doi: 10.1200/JCO.2003.02.009. J Clin Oncol. 2003. PMID: 12972522
-
Isolated tumor cells in bone marrow three years after diagnosis in disease-free breast cancer patients predict unfavorable clinical outcome.Clin Cancer Res. 2004 Aug 15;10(16):5342-8. doi: 10.1158/1078-0432.CCR-04-0245. Clin Cancer Res. 2004. PMID: 15328170
-
The persistence of isolated tumor cells in bone marrow from patients with breast carcinoma predicts an increased risk for recurrence.Cancer. 2005 Mar 1;103(5):884-91. doi: 10.1002/cncr.20834. Cancer. 2005. PMID: 15666325
-
Detection of isolated tumor cells in BM from breast-cancer patients: significance of anterior and posterior iliac crest aspirations and the number of mononuclear cells analyzed.Cytotherapy. 2003;5(1):40-5. doi: 10.1080/14653240310000065. Cytotherapy. 2003. PMID: 12745581
-
Cytomorphological evaluation of non-haematopoietic malignancies metastasizing to the bone marrow.Am J Blood Res. 2023 Feb 15;13(1):1-11. eCollection 2023. Am J Blood Res. 2023. PMID: 36937461 Free PMC article. Review.
Cited by
-
Prognostic value of PAM50 and risk of recurrence score in patients with early-stage breast cancer with long-term follow-up.Breast Cancer Res. 2017 Nov 14;19(1):120. doi: 10.1186/s13058-017-0911-9. Breast Cancer Res. 2017. PMID: 29137653 Free PMC article.
-
Individual and combined effects of DNA methylation and copy number alterations on miRNA expression in breast tumors.Genome Biol. 2013 Nov 20;14(11):R126. doi: 10.1186/gb-2013-14-11-r126. Genome Biol. 2013. PMID: 24257477 Free PMC article.
-
Adjuvant Bisphosphonate Therapy in Postmenopausal Breast Cancer.Curr Treat Options Oncol. 2018 Mar 12;19(4):18. doi: 10.1007/s11864-018-0535-z. Curr Treat Options Oncol. 2018. PMID: 29527635 Review.
-
The clinical impact of mean vessel size and solidity in breast carcinoma patients.PLoS One. 2013 Oct 11;8(10):e75954. doi: 10.1371/journal.pone.0075954. eCollection 2013. PLoS One. 2013. PMID: 24146798 Free PMC article.
-
Serum N-glycan analysis in breast cancer patients--Relation to tumour biology and clinical outcome.Mol Oncol. 2016 Jan;10(1):59-72. doi: 10.1016/j.molonc.2015.08.002. Epub 2015 Aug 19. Mol Oncol. 2016. PMID: 26321095 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Medical
Research Materials
Miscellaneous