Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1995:707:69-76.
doi: 10.1016/0021-9673(94)01276-k.

Electrochromatographic separation of proteins

S K Basak  1 , A Velayudhan, K Kohlmann, M R Ladisch
Collaborators, Affiliations

Electrochromatographic separation of proteins

S K Basak et al. J Chromatogr A. 1995.

Abstract

We have developed a modified electrochromatography system which minimizes Joule heating at electric field strengths up to 125 V/cm. A non-linear equilibrium model is described which incorporates electrophoretic mobility, hydrodynamic flow velocity, and an electrically induced concentration polarization at the surface of the stationary phase. This model is able to provide useful estimates of protein retention time and velocity in a column packed with Sephadex gel and subjected to an electric field. A correlation of electrophoretic mobility of peptide and proteins with respect to their charge, molecular mass, and asymmetry enables the selection of solute target molecules for electrochromatographic separations. Good separation of protein mixtures have been obtained.

PubMed Disclaimer

Similar articles

See all similar articles

Publication types

MeSH terms

LinkOut - more resources