Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2001 Jan 16;98(2):629-34.
doi: 10.1073/pnas.98.2.629. Epub 2001 Jan 9.

Serological detection of cutaneous T-cell lymphoma-associated antigens

S Eichmuller  1 , D Usener, R Dummer, A Stein, D Thiel, D Schadendorf
Affiliations
Comparative Study

Serological detection of cutaneous T-cell lymphoma-associated antigens

S Eichmuller et al. Proc Natl Acad Sci U S A. .

Abstract

Cutaneous T-cell lymphomas (CTCL) are a group of skin neoplasms that originate from T lymphocytes and are difficult to treat in advanced stages. The present study is aimed at the identification of tumor-specific antigens from a human testis cDNA library using human sera known as the SEREX (serological identification of recombinantly expressed genes) approach. A cDNA library from normal testicle tissue was prepared and approximately 2 million recombinants were screened with sera from Sézary Syndrome and Mycosis fungoides patients. A total of 28 positive clones belonging to 15 different genes/ORFs were identified, including five hitherto unknown sequences. Whereas control sera did not react with most clones, 11-71% sera from CTCL patients were reactive against the identified clones. Expression analysis on 28 normal control and 17 CTCL tissues by reverse transcription-PCR (RT-PCR) and Northern blotting revealed seven ubiquitously distributed antigens, six differentially expressed antigens (several normal tissues were positive), and two tumor-specific antigens that were expressed only in testis and tumor tissues: (i) A SCP-1-like sequence, which has already been detected in various tumors, has been found in one CTCL tumor and four sera of CTCL patients reacted with various SCP-1-like clones and (ii) a new sequence named cTAGE-1 (CTCL-associated antigen 1) was detected in 35% of CTCL tumor tissues and sera of 6/18 patients reacted with this clone. The present study unravels CTCL-associated antigens independent of the T-cell receptor. The SCP-1-like gene and cTAGE-1 were shown to be immunogenic and immunologically tumor-specific and may therefore be candidates for immunotherapy targeting CTCL.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Burg G, Kempf W, Haeffner A, Nestle F, Hess-Schmid M, Doebbeling U, Mueller B, Dummer R. Curr Probl Dermatol. 1997;9:137–204.
    1. Willemze R, Kerl H, Sterry W, Berti E, Cerroni L, Chimenti S, Diaz Perez J L, Geerts M L, Goos M, Knobler R, et al. Blood. 1997;90:354–371. - PubMed
    1. Jahn S, Asadullah K, Walden P, Sterry W. Immunol Today. 1998;19:100–103. - PubMed
    1. Weiss L M, Hu E, Wood G S, Moulds C, Cleary M L, Warnke R, Sklar J. N Engl J Med. 1985;313:539–544. - PubMed
    1. Dreno B, Claudy A, Meynadier J, Verret J, Souteyrand P, Ortonne J, Kalis B, Godefroy W, Beerblock K, Thill L. Br J Dermatol. 1991;125:456–459. - PubMed

Publication types

MeSH terms