Isolation and characterization of a new human breast cancer cell line, KPL-4, expressing the Erb B family receptors and interleukin-6
- PMID: 10070858
- PMCID: PMC2362677
- DOI: 10.1038/sj.bjc.6690114
Isolation and characterization of a new human breast cancer cell line, KPL-4, expressing the Erb B family receptors and interleukin-6
Abstract
A new human breast cancer cell line, KPL-4, was recently isolated from the malignant pleural effusion of a breast cancer patient with an inflammatory skin metastasis. This cell line can be cultured under serum-free conditions and is tumorigenic in female athymic nude mice. Flow cytometric analysis revealed the expression of Erb B-1, -2 and -3. Dot blot hybridization showed a 15-fold amplification of the erb B-2. Reverse transcription-polymerase chain reaction analysis showed a detectable level of mRNA expression of all the Erb B family receptors. In addition, all the receptors were autophosphorylated under a serum-supplemented condition. Unexpectedly, transplanted KPL-4 tumours induced cachexia of recipient mice. A high concentration of interleukin-6 (IL-6) was detected in both the culture medium and the serum of mice. The weight of tumours significantly correlated with the serum IL-6 level. The antiproliferative effect of a humanized anti-Erb B-2 monoclonal antibody, rhuMAbHER2, was investigated. This antibody significantly inhibited the growth of KPL-4 cells in vitro but modestly in vivo. Loss of mouse body weight was partly reversed by rhuMAbHER2. These findings suggest that KPL-4 cells may be useful in the development of new strategies against breast cancer overexpressing the Erb B family receptors and against IL-6-induced cachexia.
Similar articles
-
A new human breast cancer cell line, KPL-1 secretes tumour-associated antigens and grows rapidly in female athymic nude mice.Br J Cancer. 1995 Apr;71(4):845-53. doi: 10.1038/bjc.1995.163. Br J Cancer. 1995. PMID: 7710953 Free PMC article.
-
Medroxyprogesterone acetate inhibits interleukin 6 secretion from KPL-4 human breast cancer cells both in vitro and in vivo: a possible mechanism of the anticachectic effect.Br J Cancer. 1999 Feb;79(3-4):631-6. doi: 10.1038/sj.bjc.6690099. Br J Cancer. 1999. PMID: 10027341 Free PMC article.
-
Absence of endothelial cells, central necrosis, and fibrosis are associated with aggressive inflammatory breast cancer.Cancer Res. 2001 Jan 15;61(2):445-51. Cancer Res. 2001. PMID: 11212228
-
[Biochemical prognostic factors of ovarian carcinoma].Zentralbl Gynakol. 1995;117(5):243-6. Zentralbl Gynakol. 1995. PMID: 7793165 Review. German.
-
Regulation of interleukin-6 secretion from breast cancer cells and its clinical implications.Breast Cancer. 2000;7(2):124-9. doi: 10.1007/BF02967443. Breast Cancer. 2000. PMID: 11029783 Review.
Cited by
-
In vitro and in vivo models for analysis of resistance to anticancer molecular therapies.Curr Med Chem. 2014;21(14):1595-606. doi: 10.2174/09298673113209990226. Curr Med Chem. 2014. PMID: 23992330 Free PMC article. Review.
-
Ganoderma lucidum Combined with the EGFR Tyrosine Kinase Inhibitor, Erlotinib Synergize to Reduce Inflammatory Breast Cancer Progression.J Cancer. 2016 Feb 5;7(5):500-11. doi: 10.7150/jca.13599. eCollection 2016. J Cancer. 2016. PMID: 26958085 Free PMC article.
-
The HER2-directed antibody-drug conjugate DHES0815A in advanced and/or metastatic breast cancer: preclinical characterization and phase 1 trial results.Nat Commun. 2024 Jan 11;15(1):466. doi: 10.1038/s41467-023-44533-z. Nat Commun. 2024. PMID: 38212321 Free PMC article. Clinical Trial.
-
Dynamics of different-sized solid-state nanocrystals as tracers for a drug-delivery system in the interstitium of a human tumor xenograft.Breast Cancer Res. 2009;11(4):R43. doi: 10.1186/bcr2330. Epub 2009 Jul 3. Breast Cancer Res. 2009. PMID: 19575785 Free PMC article.
-
EGFR signaling promotes inflammation and cancer stem-like activity in inflammatory breast cancer.Oncotarget. 2017 Jul 4;8(40):67904-67917. doi: 10.18632/oncotarget.18958. eCollection 2017 Sep 15. Oncotarget. 2017. PMID: 28978083 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Research Materials