Isolation and characterization of a new human breast cancer cell line, KPL-4, expressing the Erb B family receptors and interleukin-6
- PMID: 10070858
- PMCID: PMC2362677
- DOI: 10.1038/sj.bjc.6690114
Isolation and characterization of a new human breast cancer cell line, KPL-4, expressing the Erb B family receptors and interleukin-6
Abstract
A new human breast cancer cell line, KPL-4, was recently isolated from the malignant pleural effusion of a breast cancer patient with an inflammatory skin metastasis. This cell line can be cultured under serum-free conditions and is tumorigenic in female athymic nude mice. Flow cytometric analysis revealed the expression of Erb B-1, -2 and -3. Dot blot hybridization showed a 15-fold amplification of the erb B-2. Reverse transcription-polymerase chain reaction analysis showed a detectable level of mRNA expression of all the Erb B family receptors. In addition, all the receptors were autophosphorylated under a serum-supplemented condition. Unexpectedly, transplanted KPL-4 tumours induced cachexia of recipient mice. A high concentration of interleukin-6 (IL-6) was detected in both the culture medium and the serum of mice. The weight of tumours significantly correlated with the serum IL-6 level. The antiproliferative effect of a humanized anti-Erb B-2 monoclonal antibody, rhuMAbHER2, was investigated. This antibody significantly inhibited the growth of KPL-4 cells in vitro but modestly in vivo. Loss of mouse body weight was partly reversed by rhuMAbHER2. These findings suggest that KPL-4 cells may be useful in the development of new strategies against breast cancer overexpressing the Erb B family receptors and against IL-6-induced cachexia.
Similar articles
-
A new human breast cancer cell line, KPL-1 secretes tumour-associated antigens and grows rapidly in female athymic nude mice.Br J Cancer. 1995 Apr;71(4):845-53. doi: 10.1038/bjc.1995.163. Br J Cancer. 1995. PMID: 7710953 Free PMC article.
-
Medroxyprogesterone acetate inhibits interleukin 6 secretion from KPL-4 human breast cancer cells both in vitro and in vivo: a possible mechanism of the anticachectic effect.Br J Cancer. 1999 Feb;79(3-4):631-6. doi: 10.1038/sj.bjc.6690099. Br J Cancer. 1999. PMID: 10027341 Free PMC article.
-
Absence of endothelial cells, central necrosis, and fibrosis are associated with aggressive inflammatory breast cancer.Cancer Res. 2001 Jan 15;61(2):445-51. Cancer Res. 2001. PMID: 11212228
-
[Biochemical prognostic factors of ovarian carcinoma].Zentralbl Gynakol. 1995;117(5):243-6. Zentralbl Gynakol. 1995. PMID: 7793165 Review. German.
-
Regulation of interleukin-6 secretion from breast cancer cells and its clinical implications.Breast Cancer. 2000;7(2):124-9. doi: 10.1007/BF02967443. Breast Cancer. 2000. PMID: 11029783 Review.
Cited by
-
Monitoring changing patterns in HER2 addiction by liquid biopsy in advanced breast cancer patients.J Exp Clin Cancer Res. 2024 Jun 29;43(1):182. doi: 10.1186/s13046-024-03105-9. J Exp Clin Cancer Res. 2024. PMID: 38951853 Free PMC article.
-
Trastuzumab/pertuzumab combination therapy stimulates antitumor responses through complement-dependent cytotoxicity and phagocytosis.JCI Insight. 2022 Mar 22;7(6):e155636. doi: 10.1172/jci.insight.155636. JCI Insight. 2022. PMID: 35167491 Free PMC article.
-
Activity of the kinesin spindle protein inhibitor ispinesib (SB-715992) in models of breast cancer.Clin Cancer Res. 2010 Jan 15;16(2):566-76. doi: 10.1158/1078-0432.CCR-09-1498. Epub 2010 Jan 12. Clin Cancer Res. 2010. PMID: 20068098 Free PMC article.
-
Ex vivo dual gene therapy using human adipocytes secreting anti-HER2 antibody on HER2-positive xenograft tumor models.Breast Cancer. 2023 Nov;30(6):1018-1027. doi: 10.1007/s12282-023-01497-8. Epub 2023 Aug 23. Breast Cancer. 2023. PMID: 37612442
-
Mode of action of pertuzumab in combination with trastuzumab plus docetaxel therapy in a HER2-positive breast cancer xenograft model.Oncol Lett. 2017 Oct;14(4):4197-4205. doi: 10.3892/ol.2017.6679. Epub 2017 Jul 26. Oncol Lett. 2017. PMID: 28959366 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Research Materials