Horatio could refer to:
- Horatio (character), a character from the play Hamlet
- Horatio (starship), a starship from the mid-22nd century
- USS Horatio (NCC-10532), a 24th century Federation Starfleet Ambassador-class starship
- ISS Horatio, mirror universe counterpart
- USS Horatio Ballantrye, a 24th century Federation Starfleet Ambassador Hardin-class starship
- USS Horatio (scout), a 24th century Federation Starfleet Venture-class scout ship
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