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Direct Force Measurements of Receptor–Ligand Interactions on Living Cells

  • Chapter
Applied Scanning Probe Methods XII

Part of the book series: NanoScience and Technology ((NANO))

Abstract

The characterization of cell adhesion between two living cells at the level of single receptor–ligand bonds is an experimental challenge. This chapter describes how the extremely sensitive method of atomic force microscopy (AFM) based force spectroscopy can be applied to living cells in order to probe for cell-to-cell or cell-to-substrate interactions mediated by single pairs of adhesion receptors. In addition, it is outlined how single-molecule AFM force spectroscopy can be used to detect physiologic changes of an adhesion receptor in a living cell. This force spectroscopy allows us to detect in living cells rapidly changing, chemokine SDF-1 triggered activation states of single VLA-4 receptors. This recently developed AFM application will allow for the detailed investigation of the integrin–chemokine crosstalk of integrin activation mechanisms and on how other adhesion receptors are modulated in health and disease. As adhesion molecules, living cells and even bacteria can be studied by single-molecule AFM force spectroscopy, this method is set to become a powerful tool that can not only be used in biophysics, but in cell biology as well as in immunology and cancer research.

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  1. Plainburgstr 8, 83457 Bayerisch Gmain, Germany

    Robert H. Eibl

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  1. Robert H. Eibl
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  1. Nanoprobe Laboratory for Bio- & Nanotechnology & Biomimetics (NLB2), Ohio State University, 201 W. 19th Ave, Columbus, Ohio 43210-1142, USA

    Bharat Bhushan

  2. Universität Münster FB 16 Physikalisches Institut, Wilhelm-Klemm-Str. 10, 48149 Münster, Germany

    Harald Fuchs

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Eibl, R.H. (2009). Direct Force Measurements of Receptor–Ligand Interactions on Living Cells. In: Bhushan, B., Fuchs, H. (eds) Applied Scanning Probe Methods XII. NanoScience and Technology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-85039-7_1

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