File:Investigation-of-Biophysical-Mechanisms-in-Gold-Nanoparticle-Mediated-Laser-Manipulation-of-Cells-pone.0124052.s006.ogv
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[edit]DescriptionInvestigation-of-Biophysical-Mechanisms-in-Gold-Nanoparticle-Mediated-Laser-Manipulation-of-Cells-pone.0124052.s006.ogv |
English: Exemplary video of increase in Fluo-4 fluorescence in the presence and absence of the calcium chelator EGTA. A fixed radiant exposure of 27 mJ/cm2 and irradiation time of 40 ms were used. EGTA is a calcium chelator which leads to a less pronounced increase in Fluo-4 fluorescence after laser manipulation. However, an intercellular calcium wave among non perforated cells is still observable. Scale bar 50 μm. |
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Source | S2 Video from Kalies S, Antonopoulos G, Rakoski M, Heinemann D, Schomaker M, Ripken T, Meyer H (2015). "Investigation of Biophysical Mechanisms in Gold Nanoparticle Mediated Laser Manipulation of Cells Using a Multimodal Holographic and Fluorescence Imaging Setup". PLOS ONE. DOI:10.1371/journal.pone.0124052. PMID 25909631. PMC: 4409398. | ||
Author | Kalies S, Antonopoulos G, Rakoski M, Heinemann D, Schomaker M, Ripken T, Meyer H | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 05:08, 14 October 2016 | 18 s, 873 × 364 (389 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Exemplary video of increase in Fluo-4 fluorescence in the presence and absence of the calcium chelator EGTA. |
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Author | Kalies S, Antonopoulos G, Rakoski M, Heinemann D, Schomaker M, Ripken T, Meyer H |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | A fixed radiant exposure of 27 mJ/cm2 and irradiation time of 40 ms were used. EGTA is a calcium chelator which leads to a less pronounced increase in Fluo-4 fluorescence after laser manipulation. However, an intercellular calcium wave among non perforated cells is still observable. Scale bar 50 μm. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2015 |